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To prevent microbial growth, egg water was supplemented with 0. Larval egg water was changed daily.
Glass capillary tubes World Precision Instruments, Inc.
Needles were clipped to achieve a bolus size of 5—10 nl, which was measured using a 0. Following injection, larvae were individually screened microscopically in glass bottom 96-well plates Greiner Bio-One, Monroe, NC to quantify the number of C.In the human fungal pathogen Candida albicans, cells exhibit heritable switching between two phenotypic states, white and opaque, which yield differences in mating, filamentous growth, and interactions with immune cells in vitro.
Egg water was changed daily. Images were processed with AxioVision Rel. For experiments performed at the University of Maine, at 4 h post-injection HPI larvae were anesthetized in Tricaine and immobilized in 0.